Recombinant:Article Title: Structural insight into ligand binding and activation of the orphan GPCR Mas1.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD
Sequencing:Article Title: Structural insight into ligand binding and activation of the orphan GPCR Mas1.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD
Polymerase Chain Reaction:Article Title: Structural insight into ligand binding and activation of the orphan GPCR Mas1.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD
Cell Culture:Article Title: Structural insight into ligand binding and activation of the orphan GPCR Mas1.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD
Protease Inhibitor:Article Title: Structural insight into ligand binding and activation of the orphan GPCR Mas1.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD
Modification:Article Title: Structural insight into ligand binding and activation of the orphan GPCR Mas1.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD
Cloning:Article Title: Structural insight into ligand binding and activation of the orphan GPCR Mas1.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD
Software:Article Title: Structural insight into ligand binding and activation of the orphan GPCR Mas1.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD
Cytometry:Article Title: Structural insight into ligand binding and activation of the orphan GPCR Mas1.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models E. coli OmniMAX 2 T1 Invitrogen Cat# C854003 E. coli DH10B Invitrogen Cat# EC0113 Spodoptera frugiperda (Sf9) Expression systems Cat#94-001 F HEK293T ATCC Recombinant DNA pFastBac BRIL-Mas1 This study pcDNA6 Mas1 This study N/A Antibodies N/A N/A N/A Oligonucleotides and other sequence-based reagents PCR primers Tsingke Chemicals, enzymes and other reagents AR234958 Chemical Department Synthesis NPFF tgpeptide Synthesis Ang 1–7 TargetMol Cat# T7399 ESF921 insect cell culture medium Expression system Cat# 96-001- 20 Protease Inhibitor Cocktail TargetMol Cat# C0001 Lauryl Maltose Neopentyl Glycol Anatrace Cat# NG310 Cholesteryl Hemisuccinate Tris Salt Anatrace Cat# CH210 Glyco-diosgenin Anatrace Cat# GDN101 Dulbecco’s Modified Eagle Medium (DMEM) Cytiva Cat# SH30243.01 Fetal Bovine Serum ExCell Cat# FSP500 Lipofectamine 2000 Invitrogen Cat# 11668019 Opti-MEM Gibco Cat# 11058021 ClonExpress II One Step Cloning Kit Vazyme Cat#112-01/02 Apyrase Sigma Cat#9000-957 TALON Metal Affinity Resin TAKARA Cat#635501 Software CryoSPARC https://cryosparc.com/ Version 4.5.1 MotionCor2 https://msg.ucsf.edu/software Version 1.6.4 PHENIX https://phenix-online.org/ Version 1.21.1- 5286 Reagent/resource Reference or source Identifier or catalog number COOT www.2.mrc-lmb.cam.ac.uk/ personal/pemsley/coot/ Version 0.9.8.93 UCSF Chimera https://www.cgl.ucsf.edu/ chimera/ Version 1.17.3 UCSF ChimeraX https://www.cgl.ucsf.edu/ chimerax/ Version 1.7.1 PyMOL https://pymol.org/ Version 2.5 GraphPad Prism https://www.graphpad.com/ scientific-software/prism/ Version 8.0.1 AlphaFold3 https://alphafoldserver.com Other EnVision plate reader PerkinElmer BD BiosciencesFlow Cytometer BD
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